SCIEX Lunch Seminar
LC-MS Monitoring of Biopharmaceuticals and ADCs – Simple and Fast Routine Characterisation with dedicated Hard- and Software
Join SCIEX for a lunch seminar on Day One of World ADC Berlin*. You’ll find out:
- How high resolution LC-MS/MS technology can be accessed by all levels of users – from the MS specialist to non-expert users
- How quality attributes of biotherapeutics can be monitored easily and with confidence using the power of high resolution LC-MS/MS
- How dedicated software can support the development of ADCs and other biotherapeutics
*You must be registered for the conference in order to attend the lunch seminar.
A rapidly emerging trend is the application of mass spectrometry (MS) as a key application for identification and tracking of biopharmaceutical quality attributes such as glycosylation, oxidation or drug load. A particular challenge in implementing mass spectrometry as the technique of choice has been the complexity of the analysis and the processing of the data obtained.
Here, a dedicated high resolution MS instrument focusing on ease-of-use also for non-mass-spec experts combined with simple and fast processing software especially supporting the development of biopharmaceuticals, is demonstrated. Different monoclonal antibodies and ADC biotherapeutics were analysed on intact level using reversed phase liquid chromatography (RPLC) coupled with MS. Quality attributes were identified and tracked across different batches of products and drug-antibody-ratios were determined.
Furthermore, samples were digested into peptides and analysed on peptide level using RPLC-MS. A subset of samples underwent stress conditions in order to monitor the tendency of modification for different peptides /antibody sites. Finally, samples were spiked with varying levels of known peptide to interrogate the ability of this method to detect new components in an untargeted manner. This approach could be used for flagging of degradation of drug and linker on drug-linked peptides compared to a control sample.
All data was acquired using easy-to-use high resolution time of flight MS instrumentation and processed using on single dedicated biopharmaceutical software to detect and track sample components including simple data output.